Absolutely. In fact our tour guides would be disappointed if you didn't ask questions. Our tours are for curious people. It's completely natural to have questions about cultures you may not be so familiar with.  And to be honest we'd rather you asked than went away still wondering or guessing the answer, so don't be shy, no-one will think less of you for asking.  More often your question is something another person in the group is dying to know the answer to!